Protein_Domain

Part:BBa_K2835003:Design

Designed by: Els Alsema, Marit Möller   Group: iGEM18_Stockholm   (2018-09-11)


His-tagged laccase from Trametes versicolor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 544
    Illegal BglII site found at 1240
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part does not contain a start codon. It is a protein domain that can be fused to other domains, e.g. a signal peptide. In our project, this part was inserted into the Pichia vector pPICZα A, which includes a secretion signal from Saccharomyces cerevisiae (for secretion into the medium).

6xHis-tag added at the N-terminal.

Source

Laccase gene (lcc2) from Trametes versicolor.

References